Location of features

MCS: 12-89
TurboGFP-dest1
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Last amino acid in TurboGFP: 790-792
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 808-930
Stop codon: 928-930
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 939-973
HBB intron 2: 974-1823
First 233 bp of HBB exon 3: 1824-2057
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2199-2204 & 2228-2233
mRNA 3' ends: 2237 & 2249
f1 single-strand DNA origin: 2296-2751
Bacterial promoter for expression of Kan^r gene
-35 region: 2813-2818
-10 region: 2836-2841
Transcription start point: 2848
SV40 origin of replication: 3092-3227
SV40 early promoter
Enhancer (72-bp tandem repeats): 2925-2996 & 2997-3068
21-bp repeats: 3072-3092, 3093-3113 & 3115-3135
Early promoter element: 3148-3154
Major transcription start points: 3144, 3182, 3188 & 3193
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3276-3278
Stop codon: 4068-4070
G->A mutation to remove Pst I site: 3458
C->A (Arg to Ser) mutation to remove BssH II site: 3804
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4306-4311 & 4319-4324
pUC plasmid replication origin: 4655-5298