Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
Turbo-RFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1528-1533 & 1557-1562
mRNA 3' ends: 1566 & 1578
f1 single-strand DNA origin: 1625-2080
Bacterial promoter for expression of Kan^r gene
-35 region: 2142-2147
-10 region: 2165-2170
Transcription start point: 2177
SV40 origin of replication: 2421-2556
SV40 early promoter
Enhancer (72-bp tandem repeats): 2254-2325 & 2326-2397
21-bp repeats: 2401-2421, 2422-2442 & 2444-2464
Early promoter element: 2477-2483
Major transcription start points: 2473, 2511, 2517 & 2522
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2605-2607
Stop codon: 3397-3399
G->A mutation to remove Pst I site: 2787
C->A (Arg to Ser) mutation to remove BssH II site: 3133
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3635-3640 & 3648-3653
pUC plasmid replication origin: 3984-4627