| TurboRFP | BspE I | Bgl II | Sac I | EcoR I | Sal I | Kpn I | Apa I | BamH I | Stops | |||||||||||||||||||||||
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| Xho I | Hind III | Pst I | Sac II | Xba I# | ||||||||||||||||||||||||||||
| CAC.AGA. | GAT.GAA. | TCC.GGA. | CTC. | AGA.T | CT | .C | GA.G | CT.C | AA.GCT.T | C | G.AAT.T | C | T.GCA. | G | TC.GAC | .GGT.A | CC. | GC | G.G | G | C.CC | G. | GG | A.TCC. | ACC.GGA. | TC | T.AG | A. | TAA. | C | TG.A | TC.A |
# – sites are blocked by dam methylation. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam- host and make fresh DNA.