Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TurboRFP
Start codon (ATG): 613-615
Stop codon: 1324-1326
Last amino acid in TurboRFP: 1303-1305
MCS: 1306-1392
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1532-1537 & 1561-1566
mRNA 3' ends: 1570 & 1582
f1 single-strand DNA origin: 1629-2084
Bacterial promoter for expression of Kan^r gene
-35 region: 2146-2151
-10 region: 2169-2174
Transcription start point: 2181
SV40 origin of replication: 2425-2560
SV40 early promoter
Enhancer (72-bp tandem repeats): 2258-2329 & 2330-2401
21-bp repeats: 2405-2425, 2426-2446 & 2448-2468
Early promoter element: 2481-2487
Major transcription start points: 2477, 2515, 2521 & 2526
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2609-2611
Stop codon: 3401-3403
G->A mutation to remove Pst I site: 2791
C->A (Arg to Ser) mutation to remove BssH II site: 3137
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3639-3644 & 3652-3657
pUC plasmid replication origin: 3988-4631