Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboGFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboGFP coding sequence (ATG): 705-707
Stop codon: 1401-1403
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1557-1562 & 1586-1591
mRNA 3' ends: 1595 & 1607
f1 single-strand DNA origin: 1654-2109
Bacterial promoter for expression of Kan^r gene
-35 region: 2171-2176
-10 region: 2194-2199
Transcription start point: 2206
SV40 origin of replication: 2450-2585
SV40 early promoter
Enhancer (72-bp tandem repeats): 2283-2354 & 2355-2426
21-bp repeats: 2430-2450, 2451-2471 & 2473-2493
Early promoter element: 2506-2512
Major transcription start points: 2502, 2540, 2546 & 2551
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2634-2636
Stop codon: 3426-3428
G->A mutation to remove Pst I site: 2816
C->A (Arg to Ser) mutation to remove BssH II site: 3162
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3664-3669 & 3677-3682
pUC plasmid replication origin: 4013-4656