Location of features

MCS: 12-89
TurboGFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 793-795
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 949-954 & 978-983
mRNA 3' ends: 987 & 999
f1 single-strand DNA origin: 1046-1501
Bacterial promoter for expression of Kan^r gene
-35 region: 1563-1568
-10 region: 1586-1591
Transcription start point: 1598
SV40 origin of replication: 1842-1977
SV40 early promoter
Enhancer (72-bp tandem repeats): 1675-1746 & 1747-1818
21-bp repeats: 1822-1842, 1843-1863 & 1865-1885
Early promoter element: 1898-1904
Major transcription start points: 1894, 1932, 1938 & 1943
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2026-2028
Stop codon: 2818-2820
G->A mutation to remove Pst I site: 2208
C->A (Arg to Ser) mutation to remove BssH II site: 2554
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3056-3061 & 3069-3074
pUC plasmid replication origin: 3405-4048