Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboGFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1375-1377
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1531-1536 & 1560-1565
mRNA 3' ends: 1569 & 1581
f1 single-strand DNA origin: 1628-2083
Bacterial promoter for expression of Kan^r gene
-35 region: 2145-2150
-10 region: 2168-2173
Transcription start point: 2180
SV40 origin of replication: 2424-2559
SV40 early promoter
Enhancer (72-bp tandem repeats): 2257-2328 & 2329-2400
21-bp repeats: 2404-2424, 2425-2445 & 2447-2467
Early promoter element: 2480-2486
Major transcription start points: 2476, 2514, 2520 & 2525
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2608-2610
Stop codon: 3400-3402
G->A mutation to remove Pst I site: 2790
C->A (Arg to Ser) mutation to remove BssH II site: 3136
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3638-3643 & 3651-3656
pUC plasmid replication origin: 3987-4630