Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboGFP
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1378-1380
Last amino acid in TurboGFP: 1306-1308
MCS: 1309-1394
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1520-1525 & 1549-1554
mRNA 3' ends: 1558 & 1570
f1 single-strand DNA origin: 1617-2072
Bacterial promoter for expression of Kan^r gene
-35 region: 2134-2139
-10 region: 2157-2162
Transcription start point: 2169
SV40 origin of replication: 2413-2548
SV40 early promoter
Enhancer (72-bp tandem repeats): 2246-2317 & 2318-2389
21-bp repeats: 2393-2413, 2414-2434 & 2436-2456
Early promoter element: 2469-2475
Major transcription start points: 2465, 2503, 2509 & 2514
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2597-2599
Stop codon: 3389-3391
G->A mutation to remove Pst I site: 2779
C->A (Arg to Ser) mutation to remove BssH II site: 3125
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3627-3632 & 3640-3645
pUC plasmid replication origin: 3976-4619