Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboFP602-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboFP602 coding sequence (ATG): 705-707
Stop codon: 1410-1412
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1566-1571 & 1595-1600
mRNA 3' ends: 1604 & 1616
f1 single-strand DNA origin: 1663-2118
Bacterial promoter for expression of Kan^r gene
-35 region: 2180-2185
-10 region: 2203-2208
Transcription start point: 2215
SV40 origin of replication: 2459-2594
SV40 early promoter
Enhancer (72-bp tandem repeats): 2292-2363 & 2364-2435
21-bp repeats: 2439-2459, 2460-2480 & 2482-2502
Early promoter element: 2515-2521
Major transcription start points: 2511, 2549, 2555 & 2560
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2643-2645
Stop codon: 3435-3437
G->A mutation to remove Pst I site: 2825
C->A (Arg to Ser) mutation to remove BssH II site: 3171
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3673-3678 & 3686-3691
pUC plasmid replication origin: 4022-4665