| Katushka2S | BspE I | Xho I | Hind III | Pst I | Kpn I | Apa I | BamH I | Stops | |||||||||||||||||||||||||||||
| Bgl II | Sac I | EcoR I | Sal I | Sac II | Sma I/Xma I | Xba I# | Bcl I#* | ||||||||||||||||||||||||||||||
| ... | TCC.GGA | .CTC | . | AGA.T | CT | .C | GA.G | CT.C | AA.GCT.T | C | G.AAT.T | C | T.GCA. | G | TC.GAC | . | GGT.A | CC | .GC | G.G | G | C.CC | G. | GG | A.TCC | .ACC.GGA | . | TC | T.AG | A | . | TAA | .C | TG.A | TC.A | ... | |
* – not unique site.
# – sites are blocked by dam methylation. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam- host and make fresh DNA.