Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
Katushka2S
Start codon (ATG): 613-615
Stop codon: 1396-1398
Last amino acid in Katushka2S: 1315-1317
MCS: 1318-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1538-1543 & 1567-1572
mRNA 3' ends: 1576 & 1588
f1 single-strand DNA origin: 1635-2090
Bacterial promoter for expression of Kan^r gene
-35 region: 2152-2157
-10 region: 2175-2180
Transcription start point: 2187
SV40 origin of replication: 2431-2566
SV40 early promoter
Enhancer (72-bp tandem repeats): 2264-2335 & 2336-2407
21-bp repeats: 2411-2431, 2432-2452 & 2454-2474
Early promoter element: 2487-2493
Major transcription start points: 2483, 2521, 2527 & 2532
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2615-2617
Stop codon: 3407-3409
G->A mutation to remove Pst I site: 2797
C->A (Arg to Ser) mutation to remove BssH II site: 3143
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3645-3650 & 3658-3663
pUC plasmid replication origin: 3994-4637