Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
HyPer-dMito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal 1 (MLS-1): 597-689
Mitochondrial localization signal 2 (MLS-2): 690-782
Start of HyPer coding sequence: 798-800
Stop codon: 2229-2231
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2385-2390 & 2414-2419
mRNA 3' ends: 2423 & 2435
f1 single-strand DNA origin: 2482-2937
Bacterial promoter for expression of Kan^r gene
-35 region: 2999-3004
-10 region: 3022-3027
Transcription start point: 3034
SV40 origin of replication: 3278-3413
SV40 early promoter
Enhancer (72-bp tandem repeats): 3111-3182 & 3183-3254
21-bp repeats: 3258-3278, 3279-3299 & 3301-3321
Early promoter element: 3334-3340
Major transcription start points: 3330, 3368, 3374 & 3379
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3462-3464
Stop codon: 4254-4256
G->A mutation to remove Pst I site: 3644
C->A (Arg to Ser) mutation to remove BssH II site: 3990
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4492-4497 & 4505-4510
pUC plasmid replication origin: 4841-5484