Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
HyPer
Kozak consensus translation initiation site: 596-606
Start codon (ATG): 603-605
Stop codon: 2037-2039
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2238-2243 & 2267-2272
mRNA 3' ends: 2276 & 2288
f1 single-strand DNA origin: 2335-2790
Bacterial promoter for expression of Kan^r gene
-35 region: 2852-2857
-10 region: 2875-2880
Transcription start point: 2887
SV40 origin of replication: 3131-3266
SV40 early promoter
Enhancer (72-bp tandem repeats): 2964-3035 & 3036-3107
21-bp repeats: 3111-3131, 3132-3152 & 3154-3174
Early promoter element: 3187-3193
Major transcription start points: 3183, 3221, 3227 & 3232
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3315-3317
Stop codon: 4107-4109
G->A mutation to remove Pst I site: 3497
C->A (Arg to Ser) mutation to remove BssH II site: 3843
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4345-4350 & 4358-4363
pUC plasmid replication origin: 4694-5337