Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
FusionRed-Human RhoB GTPase fusion
Start codon (ATG): 613-615
Start of FusionRed coding sequence (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
c-Myc epitope: 1363-1395
Human RhoB GTPase: 1399-1977
Stop codon: 1975-1977
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2169-2174 & 2198-2203
mRNA 3' ends: 2207 & 2219
f1 single-strand DNA origin: 2266-2721
Bacterial promoter for expression of Kan^r gene
-35 region: 2783-2788
-10 region: 2806-2811
Transcription start point: 2818
SV40 origin of replication: 3062-3197
SV40 early promoter
Enhancer (72-bp tandem repeats): 2895-2966 & 2967-3038
21-bp repeats: 3042-3062, 3063-3083 & 3085-3105
Early promoter element: 3118-3124
Major transcription start points: 3114, 3152, 3158 & 3163
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3246-3248
Stop codon: 4038-4040
G->A mutation to remove Pst I site: 3428
C->A (Arg to Ser) mutation to remove BssH II site: 3774
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4276-4281 & 4289-4294
pUC plasmid replication origin: 4625-5268