Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
FusionRed
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
Ras-related protein Rab-5A: 1342-1989
Stop codon: 1987-1989
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2150-2155 & 2179-2184
mRNA 3' ends: 2188 & 2200
f1 single-strand DNA origin: 2247-2702
Bacterial promoter for expression of Kan^r gene
-35 region: 2764-2769
-10 region: 2787-2792
Transcription start point: 2799
SV40 origin of replication: 3043-3178
SV40 early promoter
Enhancer (72-bp tandem repeats): 2876-2947 & 2948-3019
21-bp repeats: 3023-3043, 3044-3064 & 3066-3086
Early promoter element: 3099-3105
Major transcription start points: 3095, 3133, 3139 & 3144
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3227-3229
Stop codon: 4019-4021
G->A mutation to remove Pst I site: 3409
C->A (Arg to Ser) mutation to remove BssH II site: 3755
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4257-4262 & 4270-4275
pUC plasmid replication origin: 4606-5249