Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PDHA1-FusionRed fusion: 606-2504
PDHA1: 606-1772
Start codon (ATG): 606-608
Last amino acid in PDHA1: 1770-1772
FusionRed: 1806-2504
Stop codon: 2502-2504
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2657-2662 & 2686-2691
mRNA 3' ends: 2695 & 2707
f1 single-strand DNA origin: 2754-3209
Bacterial promoter for expression of Kan^r gene
-35 region: 3271-3276
-10 region: 3294-3299
Transcription start point: 3306
SV40 origin of replication: 3550-3685
SV40 early promoter
Enhancer (72-bp tandem repeats): 3383-3454 & 3455-3526
21-bp repeats: 3530-3550, 3551-3571 & 3573-3593
Early promoter element: 3606-3612
Major transcription start points: 3602, 3640, 3646 & 3651
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3734-3736
Stop codon: 4526-4528
G->A mutation to remove Pst I site: 3916
C->A (Arg to Ser) mutation to remove BssH II site: 4262
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4764-4769 & 4777-4782
pUC plasmid replication origin: 5113-5756