Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Histone H2B: 657-1034
FusionRed: 1053-1748
Stop codon: 1749-1751
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1904-1909 & 1933-1938
mRNA 3' ends: 1942 & 1954
f1 single-strand DNA origin: 2001-2456
Bacterial promoter for expression of Kan^r gene
-35 region: 2518-2523
-10 region: 2541-2546
Transcription start point: 2553
SV40 origin of replication: 2797-2932
SV40 early promoter
Enhancer (72-bp tandem repeats): 2630-2701 & 2702-2773
21-bp repeats: 2777-2797, 2798-2818 & 2820-2840
Early promoter element: 2853-2859
Major transcription start points: 2849, 2887, 2893 & 2898
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2981-2983
Stop codon: 3773-3775
G->A mutation to remove Pst I site: 3163
C->A (Arg to Ser) mutation to remove BssH II site: 3509
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4011-4016 & 4024-4029
pUC plasmid replication origin: 4360-5003