Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Connexin 43-FusionRed fusion: 824-2689
Start codon: 824-826
Connexin 43: 824-1969
FusionRed: 1991-2689
Stop codon: 2687-2689
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2842-2847 & 2871-2876
mRNA 3' ends: 2880 & 2892
f1 single-strand DNA origin: 2939-3394
Bacterial promoter for expression of Kan^r gene
-35 region: 3456-3461
-10 region: 3479-3484
Transcription start point: 3491
SV40 origin of replication: 3735-3870
SV40 early promoter
Enhancer (72-bp tandem repeats): 3568-3639 & 3640-3711
21-bp repeats: 3715-3735, 3736-3756 & 3758-3778
Early promoter element: 3791-3797
Major transcription start points: 3787, 3825, 3831 & 3836
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3919-3921
Stop codon: 4711-4713
G->A mutation to remove Pst I site: 4101
C->A (Arg to Ser) mutation to remove BssH II site: 4447
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4949-4954 & 4962-4967
pUC plasmid replication origin: 5298-5941