Main properties

TurboYFP spectra

TurboYFP normalized excitation (thin line) and emission (thick line) spectra.

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CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26
Polypeptide length, aa234
Fluorescence coloryellow
Excitation maximum, nm525
Emission maximum, nm538
Quantum yield0.53
Extinction coefficient, M-1cm-1105 000
Brightness*55.7
Brightness, % of EGFP169
pKa5.9
Structuredimer
Aggregationat high concentrations
Maturation rate at 37°Csuper fast
Photostabilityhigh
Cell toxicityat high concentrations
Main advantagessuperbright and fast-maturing yellow fluorescent protein
Possible limitationsdimer, limited applicability for fusions generation; can form aggregates at high concentration