Available variants and fusions

Variant	Description	Related vector	Cat.#	Click for image
Humanized TurboGFP	TurboGFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.	pTurboGFP-C	FP511
		pTurboGFP-N	FP512
		pTurboGFP-B	FP513
		pTurboGFP-PRL	FP515
		Gateway® TurboGFP-C entry clone	FP521
		Gateway® TurboGFP-N entry clone	FP522
Destabilized TurboGFP variant (TurboGFP-dest1)	TurboGFP-dest1 is produced by fusing the initial protein with PEST amino acid sequence encoded by region 422-461 of mouse ornithine decarboxylase gene [Li et al., 1998]. This sequence targets the protein to degradation and enables a rapid protein turnover. TurboGFP-dest1 retains spectral properties of the initial protein, but has shorter half-life (approximately 2 hrs) as measured by the analysis of fluorescence intensity of cells treated with a protein synthesis inhibitor, cycloheximide. Because of rapid turnover, TurboGFP-dest1 can be used to measure changes in gene expression.	peTurboGFP-PRL-dest1	FP523
		peTurboGFP-dest1	FP524
TurboGFP-mito fusion	A mitochondrial targeting sequence (MTS) is fused to the TurboGFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria.	pTurboGFP-mito	FP517