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Samarkina ON, Popova AG, Gvozdik EY, Chkalina AV, Zvyagin IV, Rylova YV, Rudenko NV, Lusta KA, Kelmanson IV, Gorokhovatsky AY, Vinokurov LM.
Universal and rapid method for purification of GFP-like proteins by the ethanol extraction.
Protein Expr Purif. 2009 May;65(1):108-13 doi: 10.1016/j.pep.2008.11.008
pmid: 19084068
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Constructs used: pQE30-based bacterial expression vectors encoding TagCFP, TagGFP, TagYFP, TagRFP, TurboGFP, TurboRFP, Dendra2, TurboFP602 and KillerRed.
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Expression system: E. coli strain M15 (pREP4).
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Detection system: purified recombinant FPs.
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