Available variants and fusions

Variant	Description	Related vector	Cat.#	Click for image
Humanized TagGFP2	TagGFP2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.	pTagGFP2-C	FP191
		pTagGFP2-N	FP192
TagGFP2-actin fusion	Human β-actin is fused to the TagGFP2 C-terminus. When expressed in mammalian cells, this fusion provides green fluorescent labeling of β-actin in living cells.	pTagGFP2-actin	FP194
TagGFP2-tubulin fusion	Human α-tubulin is fused to the TagGFP2 C-terminus. When expressed in mammalian cells, this fusion provides green fluorescent labeling of α-tubulin in living cells.	pTagGFP2-tubulin	FP195
TagGFP2-mito fusion	A mitochondrial targeting sequence (MTS) is fused to the TagGFP2 N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria.	pTagGFP2-mito	FP197