HPLC analysis of FusionRed in comparison with selected fluorescent proteins loaded in a high concentration.

FusionRed – orange line, mKate2 – black line, mNeptune – gray line. Proteins were loaded at a concentration of 10 mg/ml. While HPLC demonstrates reversible dimerization of mKate2 and reveals a dimeric character for the mKate derivative, far-red fluorescent protein mNeptune [Lin et al., 2009], FusionRed behaves as a pure monomer. Data from Shemiakina et al., 2012