Case12 normalized excitation (blue) and emission (green) spectra without Ca2+ (thin line) and in the presence of 1 mM of Ca2+ (thick line).
| CHARACTERISTIC | |
|---|---|
|
| |
| Emission maximum, nm | 516 |
| Excitation maximum, nm | 491 |
| Fluorescence color | green |
| Polypeptide length, aa | 415 |
| Molecular weight, kDa | 46.4 |
| Specificity | Ca2+ |
| Kd for Ca2+ | 1 μM |
| pKa | 7.2 |
| Structure | monomer |
| Aggregation | no |
| Maturation rate at 37°C | fast |