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pmKate2-annexin vector

cat.# FP321

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pmKate2-annexinFP32120 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of annexin A4

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-annexin fusion: 603-2313
mKate2: 603-1314
Start codon (ATG): 603-605
Last amino acid in mKate2: 1312-1314
Annexin A4: 1351-2313
Stop codon: 2314-2316
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2477-2482 & 2506-2511
mRNA 3' ends: 2515 & 2527
f1 single-strand DNA origin: 2574-3029
Bacterial promoter for expression of Kanr gene
-35 region: 3091-3096
-10 region: 3114-3119
Transcription start point: 3126
SV40 origin of replication: 3370-3505
SV40 early promoter
Enhancer (72-bp tandem repeats): 3203-3274 & 3275-3346
21-bp repeats: 3350-3370, 3371-3391 & 3393-3413
Early promoter element: 3426-3432
Major transcription start points: 3422, 3460, 3466 & 3471
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3554-3556
Stop codon: 4346-4348
G->A mutation to remove Pst I site: 3736
C->A (Arg to Ser) mutation to remove BssH II site: 4082
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4584-4589 & 4597-4602
pUC plasmid replication origin: 4933-5576

Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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