pTagYFP-tubulin vector

cat.# FP135

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagYFP-tubulin FP135 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagYFP Reporter codon usage mammalian Promoter for TagYFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use yellow fluorescent labeling of α-tubulin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagYFP
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 2701-2703
Last amino acid in TagYFP: 1327-1329
Tubulin: 1348-2703
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2864-2869 & 2893-2898
mRNA 3' ends: 2902 & 2914
f1 single-strand DNA origin: 2961-3416
Bacterial promoter for expression of Kan^r gene
-35 region: 3478-3483
-10 region: 3501-3506
Transcription start point: 3513
SV40 origin of replication: 3757-3892
SV40 early promoter
Enhancer (72-bp tandem repeats): 3590-3661 & 3662-3733
21-bp repeats: 3737-3757, 3758-3778 & 3780-3800
Early promoter element: 3813-3819
Major transcription start points: 3809, 3847, 3853 & 3858
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3941-3943
Stop codon: 4733-4735
G->A mutation to remove Pst I site: 4123
C->A (Arg to Ser) mutation to remove BssH II site: 4469
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4971-4976 & 4984-4989
pUC plasmid replication origin: 5320-5963