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    pTagBFP-C

pTagBFP-C vector

cat.# FP171

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pTagBFP-CFP17120 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterTagBFP
Reporter codon usagemammalian
Promoter for TagBFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use TagBFP expression in mammalian cells; generation of fusions to the TagBFP C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagBFP
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609
Stop codon: 1384-1386
Last amino acid in TagBFP: 1303-1305
MCS: 1306-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1526-1531 & 1555-1560
mRNA 3' ends: 1564 & 1576
f1 single-strand DNA origin: 1623-2078
Bacterial promoter for expression of Kanr gene
-35 region: 2140-2145
-10 region: 2163-2168
Transcription start point: 2175
SV40 origin of replication: 2419-2554
SV40 early promoter
Enhancer (72-bp tandem repeats): 2252-2323 & 2324-2395
21-bp repeats: 2399-2419, 2420-2440 & 2442-2462
Early promoter element: 2475-2481
Major transcription start points: 2471, 2509, 2515 & 2520
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2603-2605
Stop codon: 3395-3397
G->A mutation to remove Pst I site: 2785
C->A (Arg to Ser) mutation to remove BssH II site: 3131
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3633-3638 & 3646-3651
pUC plasmid replication origin: 3982-4625


Notice to Purchaser:

TagBFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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