pPA-TagRFP-tubulin vector

cat.# FP814

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPA-TagRFP-tubulin FP814 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter PA-TagRFP Reporter codon usage mammalian Promoter for PA-TagRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red fluorescent labeling of α-tubulin filaments

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 600-610
PA-TagRFP: 607-1311
Start codon (ATG): 607-609
Last amino acid in PA-TagRFP: 1309-1311
Tubulin: 1342-2697
Stop codon: 2695-2697
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2858-2863 & 2887-2892
mRNA 3' ends: 2896 & 2908
f1 single-strand DNA origin: 2955-3410
Bacterial promoter for expression of Kan^r gene
-35 region: 3472-3477
-10 region: 3495-3500
Transcription start point: 3507
SV40 origin of replication: 3751-3886
SV40 early promoter
Enhancer (72-bp tandem repeats): 3584-3655 & 3656-3727
21-bp repeats: 3731-3751, 3752-3772 & 3774-3794
Early promoter element: 3807-3813
Major transcription start points: 3803, 3841, 3847 & 3852
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3935-3937
Stop codon: 4727-4729
G->A mutation to remove Pst I site: 4117
C->A (Arg to Ser) mutation to remove BssH II site: 4463
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4965-4970 & 4978-4983
pUC plasmid replication origin: 5314-5957