pKillerRed-mem vector

cat.# FP966

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pKillerRed-mem FP966 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter KillerRed Reporter codon usage mammalian Promoter for KillerRed PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of membrane-targeted KillerRed in mammalian cells under the control of CMV promoter; source of membrane-targeted KillerRed coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KillerRed-mem fusion
Start codon (ATG): 679-681
Neuromodulin N-terminal sequence (mem): 679-738
Start of KillerRed coding sequence: 739-741
Stop codon: 1450-1452
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1606-1611 & 1635-1640
mRNA 3' ends: 1644 & 1656
f1 single-strand DNA origin: 1703-2158
Bacterial promoter for expression of Kan^r gene
-35 region: 2220-2225
-10 region: 2243-2248
Transcription start point: 2255
SV40 origin of replication: 2499-2634
SV40 early promoter
Enhancer (72-bp tandem repeats): 2332-2403 & 2404-2475
21-bp repeats: 2479-2499, 2500-2520 & 2522-2542
Early promoter element: 2555-2561
Major transcription start points: 2551, 2589, 2595 & 2600
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2683-2685
Stop codon: 3475-3477
G->A mutation to remove Pst I site: 2865
C->A (Arg to Ser) mutation to remove BssH II site: 3211
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3713-3718 & 3726-3731
pUC plasmid replication origin: 4062-4705