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    pKillerRed-N

pKillerRed-N vector

cat.# FP962

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pKillerRed-NFP96220 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterKillerRed
Reporter codon usagemammalian
Promoter for KillerRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use KillerRed expression in mammalian cells; generation of fusions to the KillerRed N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-678
KillerRed
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1552-1557 & 1581-1586
mRNA 3' ends: 1590 & 1602
f1 single-strand DNA origin: 1649-2104
Bacterial promoter for expression of Kanr gene
-35 region: 2166-2171
-10 region: 2189-2194
Transcription start point: 2201
SV40 origin of replication: 2445-2580
SV40 early promoter
Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421
21-bp repeats: 2425-2445, 2446-2466 & 2468-2488
Early promoter element: 2501-2507
Major transcription start points: 2497, 2535, 2541 & 2546
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2629-2631
Stop codon: 3421-3423
G->A mutation to remove Pst I site: 2811
C->A (Arg to Ser) mutation to remove BssH II site: 3157
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3659-3664 & 3672-3677
pUC plasmid replication origin: 4008-4651


Notice to Purchaser:

KillerRed-related materials (also referred to as "Products") are intended for research use only.

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