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    pFusionRed-C

pFusionRed-C vector

cat.# FP411

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pFusionRed-CFP41120 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
ReporterFusionRed
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use FusionRed expression in mammalian cells; generation of fusions to the FusionRed C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
FusionRed
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1399-1401
Last amino acid in FusionRed: 1306-1308
MCS: 1321-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1541-1546 & 1570-1575
mRNA 3' ends: 1579 & 1591
f1 single-strand DNA origin: 1638-2093
Bacterial promoter for expression of Kanr gene
-35 region: 2155-2160
-10 region: 2178-2183
Transcription start point: 2190
SV40 origin of replication: 2434-2569
SV40 early promoter
Enhancer (72-bp tandem repeats): 2267-2338 & 2339-2410
21-bp repeats: 2414-2434, 2435-2455 & 2457-2477
Early promoter element: 2490-2496
Major transcription start points: 2486, 2524, 2530 & 2535
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2618-2620
Stop codon: 3410-3412
G->A mutation to remove Pst I site: 2800
C->A (Arg to Ser) mutation to remove BssH II site: 3146
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3648-3653 & 3661-3666
pUC plasmid replication origin: 3997-4640


Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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