TagFP635

Far-red fluorescent protein TagFP635

Newer version is available, please see mKate2 description.

 

- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling

Performance and use

TagFP635 can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagFP635 expression vectors produce bright fluorescence in 12-14 hrs after transfection. No cell toxic effects and visible protein aggregation are observed.

TagFP use for cell labeling.

Transiently transfected mamalian cells expressing TagFP635.

Image of Phoenix Eco cells is from Shcherbo et al., 2007; image of a HeLa cell was kindly provided by Michael W. Davidson (Florida State University).

TagFP635 performance in fusions has been demonstrated in α-actinin, vinculin, zyxin, β-actin, α-tubulin, and other models.

TagFP635 use for protein labeling in mammalian cells.

Microscopic images of HeLa cells transiently transfected with TagFP635-tagged fusions. Arrows indicate gap junction.

Images were kindly provided by Michael W. Davidson (Florida State University).

TagFP635 can be used in multicolor labeling applications with blue, cyan, green, yellow, and red (orange) fluorescent dyes.

TagFP635 use in multicolor labeling of mammalian cells.

(A) TagCFP-tagged β-actin (cyan), TagYFP-tagged α-tubulin (green), TagFP635-H2B fusion (red), and Golgi-targeted TagRFP (orange); (B) TagCFP-tagged α-tubulin (cyan), TagFP635-clathrin fusion (red), mitochondria-targeted TagYFP (green). Images were kindly provided by Michael W. Davidson (Florida State University).

References:

  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
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