TagFP635

Far-red fluorescent protein TagFP635

Newer version is available, please see mKate2 description.

 

- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling

Main properties

TagFP635 spectra

TagFP635 normalized excitation (thin line) and emission (thick line) spectra.

Download TagFP635 spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
**First value – measured at pH=8.2 [Shcherbo et al., 2007], second value – measured at pH=7.5 [Shcherbo et al., 2009].
Molecular weight, kDa27
Polypeptide length, aa237
Fluorescence colorfar-red
Excitation maximum, nm588
Emission maximum, nm635
Quantum yield0.33   /   0.28 **
Extinction coefficient, M-1cm-145 000   /   31 500 **
Brightness*14.9   /   8.8 **
Brightness, % of EGFP45   /   26 **
pKa6.0
Structuremonomer
Aggregationno
Maturation rate at 37°Cfast
Photostabilityhigh
Cell toxicitynot observed
Main advantagesmonomeric far-red fluorescent protein, high photostability

Gel-filtration of TurboGFP (dimer, green line), EGFP (monomer, blue line), and TagFP635 (monomer, red line).

Image from Shcherbo et al., 2007.

References:

  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
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