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    KillerOrange

Genetically-encoded photosensitizer KillerOrange

- Blue and green light-induced production of reactive oxygen species
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required for chromophore maturation
- Not toxic before activation by blue or green light irradiation
- Recommended for selective light-induced protein inactivation and cell killing


Available variants and fusions
VariantDescription Related vector Cat.#
Humanized KillerOrange KillerOrange is developed on the basis of the KillerRed [Bulina et al., 2006]. Its codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.

Mammalian expression vectors allowing fusions to the KillerRed C-terminus / N-terminus respectively
pKillerOrange-C FP221
pKillerOrange-N FP222
KillerOrange-dMito fusion Mitochondrial targeting sequence (MTS) was derived from subunit VIII precursor of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. Two MTS were fused to KillerOrange N-terminus. When expressed in mammalian cells, this variant is localized in mitochondria. pKillerOrange-dMito FP224


References:

  • Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA. A genetically encoded photosensitizer. Nat Biotechnol. 2006; 24 (1):95-9. / pmid: 16369538
  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
  • Skene JH, Virag I. Posttranslational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP-43. J Cell Biol. 1989; 108 (2):613-24. / pmid: 2918027
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