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References citing Casper3-GR:
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Maksimov EG, Yaroshevich IA, Tsoraev GV, Sluchanko NN, Slutskaya EA, Shamborant OG, Bobik TV, Friedrich T, Stepanov AV.
A genetically encoded fluorescent temperature sensor derived from the photoactive Orange Carotenoid Protein.
Sci Rep. 2019 Jun 20;9(1):8937 doi: 10.1038/s41598-019-45421-7
pmid: 31222180
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Ryumina AP, Serebrovskaya EO, Shirmanova MV, Snopova LB, Kuznetsova MM, Turchin IV, Ignatova NI, Klementieva NV, Fradkov AF, Shakhov BE, Zagaynova EV, Lukyanov KA, Lukyanov SA.
Flavoprotein miniSOG as a genetically encoded photosensitizer for cancer cells.
Biochim Biophys Acta. 2013 Nov;1830(11):5059-67 doi: 10.1016/j.bbagen.2013.07.015
pmid: 23876295
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Registration of apoptosis, induced by miniSog-mediated oxidative stress.
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Constructs used: pCasper3-GR vector.
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Expression system: HeLa Kyoto cell line, transient expression.
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Detection system: Leica AF6000 LX imaging system based on a DMI 6000 B inverted microscope was used. A 120 W HXP short arc lamp (Osram) was a light source. Standard GFP (BP 470/40 + LP 515) and TX2 (BP 560/40 + BP 645/75) filter sets used.
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Khan MJ, Rizwan Alam M, Waldeck-Weiermair M, Karsten F, Groschner L, Riederer M, Hallström S, Rockenfeller P, Konya V, Heinemann A, Madeo F, Graier WF, Malli R.
Inhibition of autophagy rescues palmitic acid-induced necroptosis of endothelial cells.
J Biol Chem. 2012 Jun 15;287(25):21110-20 doi: 10.1074/jbc.M111.319129
pmid: 22556413
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Monitoring of lipotoxicity-induced apoptosis.
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Constructs used: pCasper3-GR vector.
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Expression system: Endothelial cells from the human umbilical vein endothelial cell-derived cell line EA.hy926, transfected using TransFast.
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Detection system: Zeiss array confocal laser scanning microscope, 488 nm laser line. Emitted light was collected at 630/75 nm.
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