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Calcium ion sensor Case12
- High dynamic range detection of intracellular Ca2+ level changes
- High selectivity and sensitivity, relatively high pH stability
- Fast maturation, high brightness of fluorescent response
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring changes in Ca2+ concentration inside living cells
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Case12 is a high dynamic range genetically encoded fluorescent sensor for direct measurement of changes of intracellular Ca2+ under various physiological and pathological conditions [Souslova et al., 2007]. The sensor is sensitive to changes of calcium concentration in a physiological range from a hundred nanomoles to micromoles with a high signal-to-noise ratio. Binding of Ca2+ is fast and reversible, allowing monitoring of high-frequency Ca2+ oscillations. In response to Ca2+ concentration rise, Case12 shows up to 12-fold increase of fluorescence brightness. Fluorescence of Case12 is characterized by single excitation/emission maxima peaked at 491/516 nm.
Case12 is recommended for monitoring change of calcium concentration inside living cells during various physiological and pathological conditions.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
The products are not available in certain countries. Please contact Evrogen or your local distributor before ordering. |
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| Case12 expression/source vectors |
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| pCase12-cyto |
FP991 |
Mammalian expression vector allowing Case12 expression in cytosol under the control of CMV promoter |
20 μg |
€ 400 / 200* |
| pCase12-mito |
FP992 |
Mammalian expression vector encoding mitochondria-targeted Case12 |
20 μg |
€ 400 / 200* |
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| Antibody against Case12 |
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Anti-GFP
antibody |
AB011 |
Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer |
100 μg |
€ 220 |
Anti-Tag(CGY)FP
antibody |
AB121 |
Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM |
100 μg |
€ 220 |
References:
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Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM.
Single fluorescent protein-based Ca2+ sensors with increased dynamic range.
BMC Biotechnol. 2007; 7 :37. / pmid: 17603870
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